A close relationship: Euwallacea fornicatus (Eichhoff, 1868) haplotype H22 (Coleoptera: Curculionidae: Scolytinae) and Ricinus communis L. (Euphorbiaceae) in Brazil

Nyeppson S. Soares; Leonardo S. Duarte; Alexandre S. Araújo; Hugo L. Rainho; Frederico Hickmann; Mateus S. L. Aurélio; Sinval Silveira-Neto; Roberto A. Zucchi; Marcoandre Savaris

doi:10.37486/2675-1305.ec08008

Authors

Nyeppson S. Soares Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil https://orcid.org/0000-0002-1296-2368
Leonardo S. Duarte Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, SP, Brazil https://orcid.org/0000-0001-6761-3010
Alexandre S. Araújo Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, SP, Brazil https://orcid.org/0000-0003-0245-763X
Hugo L. Rainho Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, SP, Brazil https://orcid.org/0000-0002-9484-0101
Frederico Hickmann The Ohio State University, Wooster, Ohio, USA https://orcid.org/0000-0002-5472-3234
Mateus S. L. Aurélio Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil https://orcid.org/0000-0002-2753-0015
Sinval Silveira-Neto Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, SP, Brazil https://orcid.org/0000-0001-7980-368X
Roberto A. Zucchi Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, SP, Brazil https://orcid.org/0000-0001-9861-7460
Marcoandre Savaris Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, SP, Brazil https://orcid.org/0000-0002-9145-6059

DOI:

https://doi.org/10.37486/2675-1305.ec08008

Keywords:

Ambrosia beetle, Breeding host, Castor bean, Polyphagous shot hole borer

Abstract

Euwallacea fornicatus (Eichhoff, 1868) (Coleoptera: Curculionidae: Scolytinae), the polyphagous shot hole borer (PSHB), is native to Asia and it has been recorded in Argentina (2023), Brazil (2024), and recently in Uruguay and Türkiye (2025), among other regions of the world. We document, for the first time, E. fornicatus on castor bean, Ricinus communis L. (Euphorbiaceae), in Brazil, which also represents the first record on this host for South America. A molecular phylogenetic analysis of PSHB from castor bean across Brazil, based on mitochondrial gene cytochrome c oxidase subunit I (COI) sequences, revealed a single haplotype (H22) in South America. We recorded PSHB on castor bean in 28 municipalities from five Brazilian states. Our results highlight the importance of including R. communis in PSHB monitoring programs.

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References

Acer, S.; Hızal, E.; Ercan, F. (2025) The First Report of Euwallacea fornicatus (Eichhoff, 1868) (Coleoptera: Scolytinae) in Türkiye with new reproductive host plant. Forestist, 75(1): 1–7. doi: 10.5152/forestist.2025.25020

Bates, D.; Maechler, M.; Bolker, B.; Walker, S.; Christensen, R. H. B.; Singmann, H.; Dai, B.; Scheipl, F.; Grothendieck, G.; Green, P., et al. (2024) Package ‘lme4’. https://github.com/lme4/lme4. Access on: 28.vi.2025.

Ceriani-Nakamurakare, E.; Johnson, A. J.; Gomez, D. F. (2023) Uncharted territories: First report of Euwallacea fornicatus (Eichhoff) in South America with new reproductive hosts records. Zootaxa, 5325(2): 289–297. doi: 10.11646/zootaxa.5325.2.10

Ceriani-Nakamurakare, E.; Gomez, D. F.; Trebino, A.; Listre, A.; Ingaramo, L.; Pilón, A. A.; Bollazzi, M. (2025) Increasing breeding host range and fast spread across Uruguay reveals the invasion potential of Euwallacea fornicatus (Coleoptera, Scolytinae) in South America. NeoBiota, 98: 247–260. doi: 10.3897/neobiota.98.147227

Covre, L. S.; Atkinson, T. H.; Johnson, A. J.; Flechtmann, C. A. H. (2024) Introduction and establishment of Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) in Brazil. Journal of Economic Entomology, 117(3): 1–6. doi: 10.1093/jee/toae081

Doyle, J. J.; Doyle, J. L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11–15.

Eskalen, A.; Gonzalez, A.; Wang, D. H.; Twizeyimana, M.; Mayorquin, J. S.; Lynch, S. C. (2012) First report of a Fusarium sp. and its vector tea shothole borer (Euwallacea fornicatus) causing Fusarium dieback on avocado in California. Plant Disease, 96(7): 1070–1070. doi: 10.1094/pdis-03-12-0276-pdn

Gadd, C. H.; Loos, C. A. (1947) The ambrosia fungus of Xyleborus fornicatus Eich. Transactions of the British Mycological Society, 31(1-2): 13–18. doi: 10.1016/s0007-1536(47)80003-8

Gallego, D.; Dios M. A. G.; Riba-Flinch, J. M.; García-Reina, A.; Galián, J.; Mas, H.; Lencina, J. L.; Zafra, M.; Henares, I.; Rodríguez, F., et al. (2025) Euwallacea similis (Ferrari), a new ambrosia beetle (Coleoptera: Curculionidae: Scolytinae) for the Iberian Peninsula, and new records on Euwallacea fornicatus (Eichhoff), Xyleborus bispinatus Eichhoff and Amasa parviseta Knížek & Smith. Zootaxa, 5673(1), 63–78. doi: 10.11646/zootaxa.5673.1.4

Gomez, D. F.; Johnson, A. J. (2019) Euwallacea fornicatus (polyphagous shot-hole borer). CABI Compendium. doi: 10.1079/cabicompendium.18360453

Gomez, D. F.; Skelton, J.; Steininger, M. S.; Stouthamer, R.; Rugman-Jones, P.; Sittichaya, W.; Rabaglia, R. J.; Hulcr, J. (2018) Species delineation within the Euwallacea fornicatus (Coleoptera: Curculionidae) complex revealed by morphometric and phylogenetic analyses. Insect Systematics & Diversity, 2(6): 1–11. doi: 10.1093/isd/ixy018

Hoang, D. T.; Chernomor, O.; Von Haeseler, A.; Minh, B. Q.; Vinh, L. S. (2018) UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution, 35(2): 518–522. doi: 10.1093/molbev/msx281

Hothorn, T.; Bretz, F.; Westfall, P.; Heiberger, R. M.; Schuetzenmeister, A.; Scheibe, S.; Hothorn, M. T. (2016) Package multcomp: Simultaneous inference in general parametric models. R package, version 1: 4–6.

Kalyaanamoorthy, S.; Minh, B. Q.; Wong, T. K.; Von Haeseler, A.; Jermiin, L. S. (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods, 14(6): 587–589. doi: 10.1038/nmeth.4285

King, C. B. R. (1941) Report of the Entomologist for 1940. Bulletin of the Tea Research Institute of Ceylon, 22: 43–49.

Lenth, R.; Singmann, H.; Love, J.; Buerkner, P.; Herve, M. (2019) Emmeans: estimated marginal means, aka least-squares means (Version 1.3.4). https://cran.r-project.org/package=emmeans. Access on: 28.iv.2025.

Li, Y.; Gu, X.; Kasson, M. T.; Bateman, C. C.; Guo, J.; Huang, Y.; Hulcr, J. (2016) Distribution, host records, and symbiotic fungi of Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) in China. Florida Entomologist, 99(4): 801–804. doi: 10.1653/024.099.0441

Liao, Y. C.; Liu, F. L.; Rugman-Jones, P. F.; Husein, D.; Liang, H. H.; Yang, Y. H.; Stouthamer, R. (2023) The Euwallacea fornicatus species complex (Coleoptera: Curculionidae); emerging economic pests of tea in Taiwan. Crop Protection, 168: 106226. doi: 10.1016/j.cropro.2023.106226

Liu, F. L.; Rugman-Jones, P.; Liao, Y. C.; Husein, D.; Liang, H. H.; Tuan, S. J.; Stouthamer, R. (2022) Seasonal dynamics of flight phenology of the Euwallacea fornicatus species complex and an associated parasitoid wasp in avocado groves in Taiwan. Journal of Economic Entomology, 115(6): 1901–1910. doi: 10.1093/jee/toac144

Mendel, Z.; Protasov, A.; Maoz, Y.; Maymon, M.; Miller, G.; Elazar, M.; Freeman, S. (2017) The role of Euwallacea nr. fornicatus (Coleoptera: Scolytinae) in the wilt syndrome of avocado trees in Israel. Phytoparasitica, 45(3): 341–359. doi: 10.1007/s12600-017-0598-6

Mendel, Z.; Protasov, A.; Sharon, M.; Zveibil, A. Y. S. B.; Yehuda, S. B.; O’Donnell, K.; Freeman, S. (2012) An Asian ambrosia beetle Euwallacea fornicatus and its novel symbiotic fungus Fusarium sp. pose a serious threat to the Israeli avocado industry. Phytoparasitica, 40(3): 235–238. doi: 10.1007/s12600-012-0223-7

Milani, M.; Cartaxo, W. V.; Vale, D. G.; Cardoso, G. D. (2011) Tecnologias Embrapa para cultura da mamoneira - BRS Energia. Embrapa Algodão. http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/921202. Access on: 28.iv.2025

Minh, B. Q.; Schmidt, H. A.; Chernomor, O.; Schrempf, D.; Woodhams, M. D.; Von Haeseler, A.; Lanfear, R. (2020) IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution, 37(5): 1530–1534. doi: 10.1093/molbev/msaa015

Mitchell, A.; Maddox, C. (2010) Bark beetles (Coleoptera: Curculionidae: Scolytinae) of importance to the Australian macadamia industry: an integrative taxonomic approach to species diagnostics. Australian Journal of Entomology, 49(2): 104–113. doi: 10.1111/j.1440-6055.2010.00746.x

Oliveira, J. M. D.; Milagres, C. H.; Ramos Filho, F. L. D. S.; Pereira, C. D. S.; Peche, P. M.; Pio, R. (2024) Performance of fig tree cultivars in the production of unripe figs for processing in Brazil. Pesquisa Agropecuária Brasileira, 59: 1–7. doi: 10.1590/s1678-3921.pab2024.v59.03785

R Core Team (2024) R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing. https://www.r-project.org. Access on: 28.iv.2025

Smith, S. M.; Cognato, A. I. (2014) A taxonomic monograph of Nearctic Scolytus Geoffroy (Coleoptera, Curculionidae, Scolytinae). ZooKeys, 450: 1–182. doi: 10.3897/zookeys.450.7452

Smith, S. M.; Gomez, D. F.; Beaver, R. A.; Hulcr, J.; Cognato, A. I. (2019) Reassessment of the species in the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) complex after the rediscovery of the "lost" type specimen. Insects 10(9): 1–11. doi: 10.3390/insects10090261

Stouthamer, R.; Rugman-Jones, P.; Thu, P. Q.; Eskalen, A.; Thibault, T.; Hulcr, J.; Wang, L. J.; Jordal, B. H.; Chen, C. Y.; Cooperband, M., et al. (2017) Tracing the origin of a cryptic invader: Phylogeography of the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) species complex. Agricultural and Forest Entomology, 19(4): 366–375. doi: 10.1111/afe.12215

Schuler, H.; Witkowski, R.; Vossenberg, B. V.; Hoppe, B.; Mittelbach, M.; Bukovinszki, T.; Schwembacher, S.; Meulengraaf, B. V.; Lange, U.; Rode, S., et al. (2023) Recent invasion and eradication of two members of the Euwallacea fornicatus species complex (Coleoptera: Curculionidae: Scolytinae) from tropical greenhouses in Europe. Biological Invasions, 25(2): 299–307. doi: 10.1007/s10530-022-02929-w

Tamura, K.; Stecher, G.; Kumar, S. (2021) MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38(2): 3022–3027. doi: 10.1093/molbev/msab120

Wang, Y.; Lu, J.; Sun, R.; Hulcr, J.; Li, Y.; Li, Y.; Gao, L. (2022) Uncovering hidden diversity within the Euwallacea fornicatus species complex in China. Entomologia Generalis, 42(4): 631–639. doi: 10.1127/entomologia/2022/1234

WFO (2025) World Flora Online. Published on the Internet. http://www.worldfloraonline.org. Access on: 28.iv.2025.

Wickham, H.; Chang, W.; Wickham, M. H. (2016) Package ggplot2. R package, version 2.1.0. https://cran.r-project.org/package=ggplot2. Access on: 28.iv.2025.